Dubois

Subscriber approaching provided by UNIV DE BURGOS Colorimetric Method for event of Sugars and Related Substances Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, and Fred. Smith Anal. Chem., 1956, 28 (3), 350-356 DOI: 10.1021/ac60111a017 Publication meet (Web): 01 may 2002 Downloaded from http://pubs.acs.org on February 12, 2009 More Ab let reveal This Article The permalink http://dx.doi.org/10.1021/ac60111a017 provides access to: Links to obligates and content cogitate to this article Copyright allowance to reproduce figures and/or text from this article Analytical Chemistry is published by the American chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Colorimetric Method for close of Sugars and Related Substances MICHEL DUBOIS, K. A. GILLES, J. K. HAMILTON, P. A. REBERS, and FRED SMITH Division o f Biochemistry, University o f Minnesota, St. Paul, M i n n . ingenuous sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hentreated with phenol and intemperate sulfuric unpleasant. The reply is sensitive and the color is stable. By practice of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In fellowship with paper partition chromatography the method is effectual for the determination of the war paint of polysaccharides and their methyl derivatives. OLORIMETRIC tests for reducing sugars and polysaccharides have been cognize for a significant time. The reagents such as 1-naphthol (33) for carbohydrates in widely distributed; benzidine for pentoses and uronic acids (27, 49,50); naphthoresorcinol for uronic acids (61 ); and resorcinol (43), naphthoresorcinol (.%), and resorcinol disulfonic acid (31) for ketoses be well-knon-n examples of colorimetric tests that may be carried out in ac id solution. Such tests as these and...If yo! u want to hold fast a full essay, order it on our website: BestEssayCheap.com

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